Shi-Fix™ Coverslips
SHI-FIX ™ COVERSLIPS and Plates for suspension cell Immunofluorescence
Do you wish suspension cell Immunofluorescence was as easy as that of adherent cells?
Now it is! Our innovative Shi-fix™ cover-slips or multi well plates allow suspension cells to be layered on them or directly grown as a monolayer. Easy, hassle-free protocol and no spin columns needed for fixing suspension cells to slides. Just add your cells to the coverslips, wait for 30 mins, wash unbound cells with PBS and proceed to immunostaining.Or continue the culture to obtain desired cell densities for suspension cell immunofluorescence.
These cover-slips can also be used for staining suspension cells for microscopy, or for fixing suspension cells for confocal microscopy. What’s more, you can also stimulate the cells while they are attached on the cover-slip, should you need to do so.
- For cells that are specially difficult to attach, we have developed the PLUS line of shi-fix coverslips.
- You can work on non-adherent cells as if they were adherent!
Simple 4 Step Protocol
1. Resuspend cultured cells in PBS
2. Add 0.2 million cells per coverslip
3. Wash with PBS to remove unbound cells
4. Proceed to immunofluorescence or continue culture of attached cells
Gallery
| SB-Shifix25 | Pack of 25 | ø 12mm x 0.17mm(#1.5) | $90 |
| SB-Shifix50 | Pack of 50 | ø 12mm x 0.17mm(#1.5) | $170 |
| SB-Shifix50PLUS | Pack of 50 | ø 15mm x 0.17mm(#1.5) | $220 |
| SB-Shifix96 | Pack of 3 | 96 well plate | $300 |
| SB-Shifix96PLUS | Pack of 3 | 96 well plate | $400 |
Terms & conditions
- Prices are for direct orders from the company. Prices through distributors may differ.
- The cost of shipping worldwide by Fedex is USD100. The product is normally delivered to your lab by FEDEX unless there are special import restrictions in your country- in that case you may need to contact the customs office of your country.
- The HS code of the product is 70179090
- Once you let us know the exact quantity you wish to order, we can send a proforma invoice for payment to our bank. Goods are dispatched from our lab within 3 working days after payment arrives in our bank. We can not process payments by credit cards.
Frequently asked questions about SHI-FIX™ products
What is the SHI-FIX™ coating material?
It is a combination of a mammalian protein and two synthetic organic compounds.
How do suspension cells adhere to SHI-FIX™ coverslips?
Hydrophilic and hydrophobic interactions between the SHI-FIX™ coating molecules and the cell membranes leads to the attachment of the cells to the coverslips.
For Which cell types are SHI-FIX™ coverslips validated ?
Jurkat, K562, U937, Daudi, PC12, Raw264.7, MOLT4, PBL culture, THP1, human PBMC, Mouse splenocytes, Mouse thymocytes, Rat splenocytes, Rat thymocytes, HL-60, T-ALL, NCI-H69
Does the of SHI-FIX™ coating stimulate the cells or alter their physiology?
As far as we know, SHI-FIX™ coatings does not stimulate the cells or alter their physiology apart from immobilizing them. We have also not observed any toxic effects on the cells.
What is the difference between SHI-FIX™ and SHI-FIX™ Plus coverslips?
Size: SHI-FIX™ coverslip is 12mm in diameter, SHI-FIX™ Plus coverslip is 15mm.
Quality: Plus line is made with high quality German glass for high resolution imaging.
Culture maintenance: Plus line is also suitable for culturing the suspension cells for a day or two prior to staining
Application: The Plus line is better for attaching cell lines which are more difficult, e.g. K562, PBMC and other blood related cell lines
What is the difference between SB-Shi-fix96 and SB-Shi-fix96Plus well plate?
SB-Shifix96 is for cell attachment followed by staining and imaging.
SB-Shifix96Plus allows you to maintain the attached cells in culture for up to 48 hours before staining.
Is it possible to do 3D culture of the cells in SHI-FIX™ coverslips?
This has not been tried.
Is there use of any human origin material to produce SHI-FIX™ products?
No
What is the shelf life of the SHI-FIX™ and SHI-FIX™ Plus coverslips?
The shelf life for both SHI-FIX™ and SHI-FIX™ plus is one year.
How do I store SHI-FIX™ products ?
Recommended storage condition is 4oC to 8oC.
How long can cells attached to SHI-FIX™ be cultured continuously?
Cells can be maintained on Shi-fix PLUS coverslips or plates for up to 5 days in culture.
Is it possible to fix microorganisms, free mitochondria, organelle membranes, and mouse embryos (with transparent body)?
Yes, it is possible to fix microorganisms like E.Coli and organelle membranes. However, mouse embryos have not yet been tried.
Is it possible to measure the metabolic activity of the attached cells?
Yes
Can the cells bound to Shi-fix can be peeled off?
This has not been tried.
How much of the cell population is lost after 3 washes?
Usually 10-20% of total cells (depending on the cell types) will be lost after 3 washes.
Is it possible to attach a mixture of adherent and suspension cells to the coverslips?
Yes. Add adherent cells to the coverslips first, wait for 30 min, then add suspension cells and wait for another 30 min before washing.
If the number of cells on the coverslips is insufficient after adding the cell suspension, can you can add the cell suspension again?
Yes
Do the cells adhere to the coverslips in a double layer ?
No. The attachment is a monolayer.
Can the Shi-Fix coverslips be applied to the study of CAR-T immune synapses?
We haven't tested yet however it is probably possible to use Shi-fix coverslips to the study of CAR-T immune synapses. Recently, we co-cultured Adherent and suspension cells , K562 and HeLa ,on the SHI-FIX™ coverslips and the cells were very healthy and growing in a monolayer (K562 cells and HeLa cells co cultured).
Have you tested the lower limit of applicable cells on SHI-FIX™ COVERSLIPS?
we have used a minimum 15000 cells per coverslip. Cells fewer than that probably need to be resuspended in a very small volume- 10-20 ul.
Are SHI-FIX ™ COVERSLIPS and Plates all sterile?
Yes, all our SHI-FIX™ products are sterile.
Can we seed cells without washing with PBS?
Yes, we can seed cells without washing with PBS but attachment of cells will be very low as the serum present in the medium interferes the cell attachment.
Publications that have used SHI-FIX™ coverslips and plates:
CXCR4-targeted PET imaging in rheumatoid arthritis: a novel approach for monitoring disease activity and therapeutic response. Han, Y., Cao, S., Liu, J. et al. EJNMMI Res 15, 10 (2025). https://doi.org/10.1186/s13550-025-01203-z
BCAT1 Associates with DNA Repair Proteins KU70 and KU80 and Contributes to Regulate DNA Repair in T-Cell Acute Lymphoblastic Leukemia (T-ALL) Valeria Tosello et al, In J. Mol. Sci. 2024, 25(24), 13571; https://doi.org/10.3390/ijms252413571
Characterization of an expanded set of assays for immunomodulatory proteins using targeted mass spectrometry Jeffrey R. Whiteaker et al, Nature Scientific Data volume 11, Article number: 682 (2024) https://doi.org/10.1038/s41597-024-03467-x
Therapeutic and prophylactic applications of cell penetrating, anti-DNA binding proteins, in particular in the context of inflammatory disease and complications (International Patent WO2023034778A1 ) James A. Campbell, Valentina DUBLJEVIC, James E. Hansen, Xiaoyong Chen, Benedette J. CUFFARI Anupama Shirali, Jiangbing Zhou WO2023034778A1 - Method of treatment - Google Patents
A peptide derived from interleukin-10 exhibits potential anticancer activity and can facilitate cell targeting of gold nanoparticles loaded with anticancer therapeutics. Chun-Chun Chang et al. Nature Communications Chemistry, volume 6, Article number: 278 (2023)https://doi.org/10.1038/s42004-023-01079-x
Synthetic lethality of drug-induced polyploidy and BCL-2 inhibition in lymphoma. Ana Portelinha et al. Nat Commun. 2023 Mar 18;14(1):1522. PMID: 36934096.
Epi-microRNA mediated metabolic reprogramming ensures affinity maturation. Rinako Nakagawa et al. bioRxiv, The Cold Spring Harbour Laboratory DOI: https://doi.org/10.1101/2023.07.31.551250
Cancer-associated fibroblasts impair the cytotoxic function of NK cells in gastric cancer by inducing ferroptosis via iron regulation. Lizhong Yao 1, Junyi Hou 1, Xiongyan Wu
MiR-155 epigenetically counteracts hypoxia for mitochondrial fitness during germinal center positive selection. Rinako Nakagawa et al. https://www.biorxiv.org/content/10.1101/2023.07.31.551250
Inhibition of NETosis by a Nuclear-Penetrating Anti-DNA Autoantibody. , ,
Carboxy-terminal dendrimers with phenylalanine for a pH-sensitive delivery system into immune cells including T cells. Hiroya Shiba et al. 2022 Apr 6;10(14):2463-2470. doi: 10.1039/d1tb01980e.
USP22 controls tumorigenicity by regulating interferon responses and the stability of the tumor suppressor PML. Lisa Kowald. Goethe University Frankfurt, Biochemistry, Chemistry and Pharmacy Doctoral Thesis 2022
Genetic Disruption of Transfer RNA Modifications in Human Cancer. Laia Coll San Martin
Targeted Mass Spectrometry Enables Quantification of Novel Pharmacodynamic Biomarkers of ATM Kinase Inhibition. Jeffrey R. Whiteaker and Tao Wang et al. Cancers 2021, 13(15), 3843; https://doi.org/10.3390/cancers13153843
Coming in and Finding Out: Blending Receptor-Targeted Delivery and Efficient Endosomal Escape in a Novel Bio-Responsive siRNA Delivery System for Gene Knockdown in Pulmonary T Cells. Kandil R, Xie Y, Heermann R, et al. Adv Ther (Weinh). 2019;2(7):1900047. doi:10.1002/adtp.201900047
Specific Targeting of Somatostatin Receptor Subtype-2 for Fluorescence-Guided Surgery Servando Hernandez Vargas et al. Clin Cancer Res July 15 2019 25 (14) 4332-4342; DOI:10.1158/1078-0432.CCR-18-3312